nexttec™ Biotechnologie GmbH - the fastest way to get PURIFIED DNA - tested millions of time

Principle:
nexttec™ 1-Step Blood (200 µl) is the easiest handling and fastest DNA purification system containing a single buffer system and a 1-Step DNA purification after lysis in 4 minutes. 

The nexttec™ 1-Step technology is a reversal of the usual, known DNA purification systems (silica membrane or magnetic beads). Proteins, detergents and low molecular weight compounds are retained by the nexttec™ sorbent.

Source:
Fresh or frozen, EDTA, Heparin blood

Lysistime:

  • 30 min to overnight

Amount of DNA:

  • Up to 10 µg

Protocol for  Isolation of  Blood (200 µl) using nexttec™ cleanPlates96:

Sample Lysis
  • Transfer 600 µl Buffer Solution B to each well of a deep-well plate.
  • Add 200 µl EDTA or heparinized blood.
  • Mix thoroughly by pipetting up and down.
  • Incubate deep-well plate 5 min on ice.
  • Centrifuge (2,000x g, 10 min), remove and discard the supernatant.
  • Add 125 µl Buffer R, 20 µl Proteinase K and 1.5 µl DTT.
  • Resuspend the pellet by pipetting up and down (3 times).
  • Incubate with shaking (56°C, 600 rpm, 30 min).
blood sample-lysis

1-Step DNA Purification

  • Transfer 100 µl of the lysate to an equilibrated nexttec™ cleanPlates96.
  • Incubate for 3 min at room temperature.
  • Centrifuge at 700x g for 1 minor apply vacuum for 1 min.

The Eluate contains the Purified DNA!!

blood 1step

Ordering Informations:

Cat. No. Article Quantity of Preparations
  nexttec™ 1-Step DNA Isolation Kit for Blood (200 µl)
  nexttec™ cleanColumns
50N. 010 nexttec™ 1-Step DNA Isolation Kit for Blood (200 µl)
10 nexttec™ cleanColumns
10
50N. 050 nexttec™ 1-Step DNA Isolation Kit for Blood (200 µl)
50 nexttec™ cleanColumns
50
50N. 250 nexttec™ 1-Step DNA Isolation Kit for Blood (200 µl)
250 nexttec™ cleanColumns
250
  nexttec™ cleanPlates96  
50N. 901 nexttec™ 1-Step DNA Isolation Kit for Blood (200 µl)
1 nexttec™ cleanPlate96
1x 96
50N. 902 nexttec™ 1-Step DNA Isolation Kit for Blood (200 µl)
2 nexttec™ cleanPlates96
2x 96
50N. 904 nexttec™ 1-Step DNA Isolation Kit for Blood (200 µl)
4 nexttec™ cleanPlates96
4x 96
50N. 924 nexttec™ 1-Step DNA Isolation Kit for Blood (200 µl)
24 nexttec™ cleanPlates96
24x 96

Protocols:

pdf50N-Blood200_2.0_nexttec-cleanColumns.pdf

pdf50N-Blood200_2.0_nexttec-cleanPlate96.pdf

 

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