nexttec™ Biotechnologie GmbH - the fastest way to get PURIFIED DNA - tested millions of time

Principle:
nexttec™ 1-Step Plant is the easiest handling and fastest DNA purification system containing a single buffer system and a 1-Step DNA purification after lysis in 4 minutes. 

The nexttec™ 1-Step technology is a reversal of the usual, known DNA purification systems (silica membrane or magnetic beads). Proteins, detergents and low molecular weight compounds are retained by the nexttec™ sorbent.

Source:

leaves tissue, plant seeds

Lysistime:

  • 30 min to overnight

Amount of DNA:

  • Up to 2,5 µg

Protocol for  Isolation of  Plant (Seed) using nexttec™ cleanPlates96:

Sample Lysis
  • Transfer up to 20 mg seed meal, grist or a single crude squashed seed to a deep-well plate.
  • Add 280 µl Buffer F, 20 µl Protease and 3 µl DTT.
  • Incubate with shaking (60°C, 750 rpm, 30 min).
  • Centrifuge the lysate (2,000x g, 3 min).
  • Use clear supernatant for DNA purification.
blood sample-lysis

1-Step DNA Purification

  • Transfer 100 µl of the lysate to an equilibrated nexttec™ cleanPlates96.
  • Incubate for 3 min at room temperature.
  • Centrifuge at 700x g for 1 min or apply vacuum for 1 min.

The Eluate contains the Purified DNA!!

blood 1step

Ordering Informations:

Cat. No. Article Quantity of Preparations
nexttec™ 1-Step DNA Isolation Kit for Plant
nexttec™ cleanColumns
47N. 010 nexttec™ 1-Step DNA Isolation Kit for Plant
10 nexttec™ cleanColumns
10
47N. 050 nexttec™ 1-Step DNA Isolation Kit for Plant
50 nexttec™ cleanColumns
50
47N. 250 nexttec™ 1-Step DNA Isolation Kit for Plant
250 nexttec™ cleanColumns
250
nexttec™ cleanPlates96
47N. 901 nexttec™ 1-Step DNA Isolation Kit for Plant
1 nexttec™ cleanPlate96
1x 96
47N. 902 nexttec™ 1-Step DNA Isolation Kit for Plant
2 nexttec™ cleanPlates96
2x 96
47N. 904 nexttec™ 1-Step DNA Isolation Kit for Plant
4 nexttec™ cleanPlates96
4x 96
47N. 924 nexttec™ 1-Step DNA Isolation Kit for Plant
24 nexttec™ cleanPlates96
24x 96

Protocols:

pdf47N-Plant_1.0_nexttec-cleanColumns.pdf

pdf47N-Plant_1.0_nexttec-cleanPlate96.pdf

 

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