nexttec™ Biotechnologie GmbH - the fastest way to get PURIFIED DNA - tested millions of time

Plasmids

Principle:
nexttec™ 1-Step Plasmid is the easiest handling and fastest DNA purification system containing a single buffer system and a 1-Step DNA purification after lysis in 4 minutes. 

The nexttec™ 1-Step technology is a reversal of the usual, known DNA purification systems (silica membrane or magnetic beads). Proteins, detergents and low molecular weight compounds are retained by the nexttec™ sorbent.

Source:
low copy, high copy plasmid from E.coli strains

Lysistime:

  • 9 min

Amount of DNA:

  • 0,8 to 2 µg

Protocol for Isolation of Plasmid using nexttec™ cleanPlates96:

Sample Lysis

  • Transfer 1 ml bacteria overnight cultures to a deep-well plate.
  • Add 130 µl Buffer P, 5 µl Protease and 3 µl Lysozyme to the cell pellet.
  • Dissolve cell pellets by pipetting up and down, close plate with an alu sealing tape.
  • Incubate for 5 min at room temperature.
  • Transfer plate to a preheated water bath (90°C).
  • Incubate for 1 min.
  • Cool down plate to room temperature.
  • Add 15 µl RNase A to each lysate.
  • Mix gently (do not vortex!).
  • Incubate for 3 min at room temperature.

 

 blood sample-lysis

1-Step DNA Purification

  • Transfer the complete lysate onto an equili-brated nexttecTM cleanPlate96 (use 1 ml or 200 µl wide bore pipette tips).
  • Incubate for 3 min at room temperature.
  • Centrifuge at 700x g for 1 min or apply vacuum for 1 min.

The Eluate contains the Purified DNA!!

 blood 1step

Ordering Informations:

Cat. No. Article Quantity of Preparations
  nexttec™ 1-Step DNA Isolation Kit for Plasmid
  nexttec™ cleanColumns
30N. 010 nexttec™ 1-Step DNA Isolation Kit for Plasmid
10 nexttec™ cleanColumns		 10
30N. 050 nexttec™ 1-Step DNA Isolation Kit for Plasmid
50 nexttec™ cleanColumns		 50
30N. 250 nexttec™ 1-Step DNA Isolation Kit for Plasmid
250 nexttec™ cleanColumns		 250
  nexttec™ cleanPlates96
30N. 901 nexttec™ 1-Step DNA Isolation Kit for Plasmid
1 nexttec™ cleanPlate96		 1x 96
30N. 902 nexttec™ 1-Step DNA Isolation Kit for Plasmid
2 nexttec™ cleanPlates96		 2x 96
30N. 904 nexttec™ 1-Step DNA Isolation Kit for Plasmid
4 nexttec™ cleanPlates96		 4x 96
30N. 924 nexttec™ 1-Step DNA Isolation Kit for Plasmid
24 nexttec™ cleanPlates96		 24x 96

Protocols:

pdf30N-Plasmid_3.0_nexttec-cleanColumns.pdf

pdf30N-Plasmid_3.0_nexttec-cleanPlate96.pdf

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