nexttec™ Biotechnologie GmbH - the fastest way to get PURIFIED DNA - tested millions of time

Principle:
nexttec™ 1-Step Bacteria is the easiest handling and fastest DNA purification system containing a single buffer system and a 1-Step DNA purification after lysis in 4 minutes. The nexttec™ 1-Step technology is a reversal of the usual, known DNA purification systems (silica membrane or magnetic beads). Proteins, detergents and low molecular weight compounds are retained by the nexttec™ sorbent.

Source:
Gram-positive or gram-negative Bacteria

Special protocols for nexttec™ cleanColumns and nexttec™ cleanPlates96  are available for:

  • Bacteria in milk
  • Detection von Pasteurella multocida spp.

Lysistime:

  • 40 min

Amount of DNA:

  • Up to 4 µg depending on the strain.

Protocol for Isolation of Bacteria using nexttec™ cleanPlates96:

Sample Lysis
  • Transfer 0.5 ml bacterial cultures (up to 1.5 OD600) to a deep-well plate.
  • Add 90 µl Buffer B, 10 µl Lysozyme and 20 µl RNaseA* to the cell pellet.
  • Resuspend cells gently by pipetting up and down.
  • Incubate with shaking (56°C, 200 rpm, 10 min.).
  • Add 90 µl SDS Solution, 10 µl Proteinase K, 2,5 µl DTT and 2 µl EDTA* to each sample.
  • Incubate with shaking (56°C, 200 rpm, 30 min.).
blood sample-lysis

1-Step DNA Purification

  • Transfer 100 µl of the lysate to an equilibrated nexttec™ cleanPlates96.
  • Incubate for 3 min at room temperature.
  • Centrifuge at 700x g for 1 min or apply vacuum for 1 min.

The Eluate contains the Purified DNA!!

blood 1step

Ordering Informations:

Cat. No. Article Quantity of Preparations
  nexttec™ 1-Step DNA Isolation Kit for Bacteria
  nexttec™ cleanColumns
20N. 010 nexttec™ 1-Step DNA Isolation Kit for Bacteria
10 nexttec™ cleanColumns
10
20N. 050 nexttec™ 1-Step DNA Isolation Kit for Bacteria
50 nexttec™ cleanColumns
50
20N. 250 nexttec™ 1-Step DNA Isolation Kit for Bacteria
250 nexttec™ cleanColumns
250
  nexttec™ cleanPlates96  
20N. 901 nexttec™ 1-Step DNA Isolation Kit for Bacteria
1 nexttec™ cleanPlate96
1x 96
20N. 902 nexttec™ 1-Step DNA Isolation Kit for Bacteria
2 nexttec™ cleanPlates96
2x 96
20N. 904 nexttec™ 1-Step DNA Isolation Kit for Bacteria
4 nexttec™ cleanPlates96
4x 96
20N. 924 nexttec™ 1-Step DNA Isolation Kit for Bacteria
24 nexttec™ cleanPlates96
24x 96

Protocols:


pdf20N-Bacteria_3.0_nexttec-cleanColumns.pdf

pdf20N-Bacteria_3.0_nexttec-cleanPlate96.pdf

pdf22N-Bacteria_milk_3.0_nexttec-cleanColumns.pdf

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