nexttec™ Biotechnologie GmbH - the fastest way to get PURIFIED DNA - tested millions of time

Principle:
nexttec™ 1-Step Bacteria is the easiest handling and fastest DNA purification system containing a single buffer system and a 1-Step DNA purification after lysis in 4 minutes. The nexttec™ 1-Step technology is a reversal of the usual, known DNA purification systems (silica membrane or magnetic beads). Proteins, detergents and low molecular weight compounds are retained by the nexttec™ sorbent.

Source:
Gram-positive or gram-negative Bacteria

Special protocols for nexttec™ cleanColumns and nexttec™ cleanPlates96  are available for:

  • Bacteria in milk
  • Detection von Pasteurella multocida spp.

Lysistime:

  • 40 min

Amount of DNA:

  • Up to 4 µg depending on the strain.

Protocol for Isolation of Bacteria using nexttec™ cleanPlates96:

Sample Lysis
  • Transfer 0.5 ml bacterial cultures (up to 1.5 OD600) to a deep-well plate.
  • Add 90 µl Buffer B, 10 µl Lysozyme and 20 µl RNaseA* to the cell pellet.
  • Resuspend cells gently by pipetting up and down.
  • Incubate with shaking (56°C, 200 rpm, 10 min.).
  • Add 90 µl SDS Solution, 10 µl Proteinase K, 2,5 µl DTT and 2 µl EDTA* to each sample.
  • Incubate with shaking (56°C, 200 rpm, 30 min.).
    blood sample-lysis

    1-Step DNA Purification

    • Transfer 100 µl of the lysate to an equilibrated nexttec™ cleanPlates96.
    • Incubate for 3 min at room temperature.
    • Centrifuge at 700x g for 1 min or apply vacuum for 1 min.

    The Eluate contains the Purified DNA!!

    blood 1step

    Ordering Informations:

    Cat. No. Article Quantity of Preparations
    nexttec™ 1-Step DNA Isolation Kit for Bacteria
    nexttec™ cleanColumns
    20N. 010 nexttec™ 1-Step DNA Isolation Kit for Bacteria
    10 nexttec™ cleanColumns
    10
    20N. 050 nexttec™ 1-Step DNA Isolation Kit for Bacteria
    50 nexttec™ cleanColumns
    50
    20N. 250 nexttec™ 1-Step DNA Isolation Kit for Bacteria
    250 nexttec™ cleanColumns
    250
    nexttec™ cleanPlates96
    20N. 901 nexttec™ 1-Step DNA Isolation Kit for Bacteria
    1 nexttec™ cleanPlate96
    1x 96
    20N. 902 nexttec™ 1-Step DNA Isolation Kit for Bacteria
    2 nexttec™ cleanPlates96
    2x 96
    20N. 904 nexttec™ 1-Step DNA Isolation Kit for Bacteria
    4 nexttec™ cleanPlates96
    4x 96
    20N. 924 nexttec™ 1-Step DNA Isolation Kit for Bacteria
    24 nexttec™ cleanPlates96
    24x 96

    Protocols:


    pdf20N-Bacteria_1.0_nexttec-cleanColumns.pdf

    pdf20N-Bacteria_1.0_nexttec-cleanPlate96.pdf

    pdf20N-BacteriaMilk_1.0_nexttec-cleanColumns.pdf

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