Tissue and Cells

nexttec™ Biotechnologie GmbH - the fastest way to get PURIFIED DNA - tested millions of time

Principle:
nexttec™ 1^-Step Tissue & Cells is the easiest handling and fastest DNA purification system containing a single buffer system and a 1^-Step DNA purification after lysis in 4 minutes.

The nexttec™ 1^-Step technology is a reversal of the usual, known DNA purification systems (silica membrane or magnetic beads). Proteins, detergents and low molecular weight compounds are retained by the nexttec™ sorbent.

Source:
Fresh or frozen tissue, cell cultures

Special protocols for nexttec™ cleanColumns and nexttec™ cleanPlates96 are available for:

Mouse-tail
Fish
Buccal swabs
Drosophila
Hair
Sperm
Forensic application (“Fingerprint”)
detailed informations: http://www.fdfproject.com.ar
Other protocols on request

Lysistime:

30 min to overnight

Amount of DNA:

up to 15 µg depending on the source

Protocol for Isolation of Mouse tail using nexttec™ cleanPlates96:

Sample Lysis

Transfer mouse tail (8–12 mg fresh weight; 4–6 mm length) to a deep-well plate.
Add 140 µl Buffer G and 10 µl Proteinase K to each sample.
Incubate the sample with shaking (56°C, 200 rpm, 30 min to overnight)

1-Step DNA Purification

Transfer 100 µl of the lysate to an equilibrated nexttec™ cleanPlate96.
Incubate for 3 min at room temperature.
Centrifuge at 700x g for 1 min or apply vacuum for 1 min.

The Eluate contains the Purified DNA!!

Ordering Informations:

Cat. No.	Article	Quantity of Preparations
	nexttec™ 1^-Step DNA Isolation Kit for Tissue & Cells
	nexttec™ cleanColumns
10N. 010	nexttec™ 1^-Step DNA Isolation Kit for Tissue & Cells 10 nexttec™ cleanColumns	10
10N. 050	nexttec™ 1^-Step DNA Isolation Kit for Tissue & Cells 50 nexttec™ cleanColumns	50
10N. 250	nexttec™ 1^-Step DNA Isolation Kit for Tissue & Cells 250 nexttec™ cleanColumns	250
	nexttec™ cleanPlates96
10N. 901	nexttec™ 1^-Step DNA Isolation Kit for Tissue & Cells 1 nexttec™ cleanPlate96	1x 96
10N. 902	nexttec™ 1^-Step DNA Isolation Kit for Tissue & Cells 2 nexttec™ cleanPlates96	2x 96
10N. 904	nexttec™ 1^-Step DNA Isolation Kit for Tissue & Cells 4 nexttec™ cleanPlates96	4x 96
10N. 924	nexttec™ 1^-Step DNA Isolation Kit for Tissue & Cells 24 nexttec™ cleanPlates96	24x 96