nexttec™ Biotechnologie GmbH - the fastest way to get PURIFIED DNA - tested millions of time

Principle:
nexttec™ 1-Step Tissue & Cells is the easiest handling and fastest DNA purification system containing a single buffer system and a 1-Step DNA purification after lysis in 4 minutes. 

The nexttec™ 1-Step technology is a reversal of the usual, known DNA purification systems (silica membrane or magnetic beads). Proteins, detergents and low molecular weight compounds are retained by the nexttec™ sorbent.

Source:
Fresh or frozen tissue, cell cultures

Special protocols for nexttec™ cleanColumns and nexttec™ cleanPlates96  are available for:

  • Mouse-tail
  • Fish
  • Buccal swabs
  • Drosophila
  • Hair
  • Sperm
  • Forensic application (“Fingerprint”)
    detailed informations: http://www.fdfproject.com.ar
  • Other protocols on request

Lysistime:

  • 30 min to overnight

Amount of DNA:

  • up to 15 µg depending on the source

Protocol for Isolation of Mouse tail using nexttec™ cleanPlates96:

Sample Lysis

  • Transfer mouse tail (8–12 mg fresh weight; 4–6 mm length) to a deep-well plate.
  • Add 140 µl Buffer G and 10 µl Proteinase K to each sample.
  • Incubate the sample with shaking (56°C, 200 rpm, 30 min to overnight)
blood sample-lysis

1-Step DNA Purification

  • Transfer 100 µl of the lysate to an equilibrated nexttec™ cleanPlate96.
  • Incubate for 3 min at room temperature.
  • Centrifuge at 700x g for 1 min or apply vacuum for 1 min.

The Eluate contains the Purified DNA!!

blood 1step

Ordering Informations:

Cat. No. Article Quantity of Preparations
nexttec™ 1-Step DNA Isolation Kit for Tissue & Cells
nexttec™ cleanColumns
10N. 010 nexttec™ 1-Step DNA Isolation Kit for Tissue & Cells
10 nexttec™ cleanColumns
10
10N. 050 nexttec™ 1-Step DNA Isolation Kit for Tissue & Cells
50 nexttec™ cleanColumns
50
10N. 250 nexttec™ 1-Step DNA Isolation Kit for Tissue & Cells
250 nexttec™ cleanColumns
250
nexttec™ cleanPlates96
10N. 901 nexttec™ 1-Step DNA Isolation Kit for Tissue & Cells
1 nexttec™ cleanPlate96
1x 96
10N. 902 nexttec™ 1-Step DNA Isolation Kit for Tissue & Cells
2 nexttec™ cleanPlates96
2x 96
10N. 904 nexttec™ 1-Step DNA Isolation Kit for Tissue & Cells
4 nexttec™ cleanPlates96
4x 96
10N. 924 nexttec™ 1-Step DNA Isolation Kit for Tissue & Cells
24 nexttec™ cleanPlates96
24x 96

Protocols:

pdf10N-BuccalSwabs_1.0_nexttec-cleanColumns.pdf

pdf10N-BuccalSwab_1.0_nexttec-cleanPlate96.pdf

pdf10N-Cigarette_Butts_1.0_nexttec-cleanColumns.pdf

pdf10N-Drosophila_1.0_nexttec-cleanColumns.pdf

pdf10N-Drosophila_1.0_nexttec-cleanPlate96.pdf

pdf10N-Fish_1.0_nexttec-cleanColumns.pdf

pdf10N-Fish_1.0_nexttec-cleanPlate96.pdf

pdf10N-Hair_1.0_nexttec-cleanColumns.pdf

pdf10N-Hair_1.0_nexttec-cleanPlate96.pdf

pdf10N-MouseTail_1.0_nexttec-cleanColumns.pdf

pdf10N-MouseTail_1.0_nexttec-cleanPlate96.pdf

pdf10N-Sperm_1.0_nexttec-cleanColumns.pdf

pdf10N-Tissue-Cells_1.0_nexttec-cleanColumns.pdf

pdf10N-Tissue-Cell_1.0_nexttec-cleanPlate96.pdf

pdf10N-TypiFix_1.0_nexttec-cleanColumns.pdf

pdf10N-TypiFix_1.0_nexttec-cleanPlate96.pdf

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