nexttec™ Biotechnologie GmbH - the fastest way to get PURIFIED DNA - tested millions of time

Principle:
nexttec™ 1-Step Blood (10 µl) is the easiest handling and fastest DNA purification system containing a single buffer system and a 1-Step DNA purification after lysis in 4 minutes.

The nexttec™ 1-Step technology is a reversal of the usual, known DNA purification systems (silica membrane or magnetic beads). Proteins, detergents and low molecular weight compounds are retained by the nexttec™ sorbent.

Source:
Fresh or frozen, EDTA, Heparin blood

Lysistime:

  • 30 min to overnight

Amount of DNA:

  • Up to 10 µg

Protocol for Isolation of Blood (10 µl) using nexttec™ cleanPlates96:

Sample Lysis
  • Transfer 10 µl samples of EDTA or heparinized blood to a deep-well plate 
  • Add 140 µl Buffer H, 10 µl Proteinase K to each sample. 
  • Incubate with shaking (60°C, 200 rpm, 2h to overnight).
blood sample-lysis

1-Step DNA Purification

  • Transfer 100 µl of the lysate to an equilibrated nexttec™ cleanPlates96.
  • Incubate for 3 min at room temperature.
  • Centrifuge at 700x g for 1 min or apply vacuum for 1 min.

The Eluate contains the Purified DNA!!

blood 1step

Ordering Informations:

Cat. No. Article Quantity of Preparations
nexttec™ 1-Step DNA Isolation Kit for Blood (10 µl)
nexttec™ cleanColumns
55N. 010 nexttec™ 1-Step DNA Isolation Kit for Blood (10 µl)
10 nexttec™ cleanColumns
10
55N. 050 nexttec™ 1-Step DNA Isolation Kit for Blood (10 µl)
50 nexttec™ cleanColumns
50
55N. 250 nexttec™ 1-Step DNA Isolation Kit for Blood (10 µl)
250 nexttec™ cleanColumns
250
nexttec™ cleanPlates96
55N. 901 nexttec™ 1-Step DNA Isolation Kit for Blood (10 µl)
1 nexttec™ cleanPlate96
1x 96
55N. 902 nexttec™ 1-Step DNA Isolation Kit for Blood (10 µl)
2 nexttec™ cleanPlates96
2x 96
55N. 904 nexttec™ 1-Step DNA Isolation Kit for Blood (10 µl)
4 nexttec™ cleanPlates96
4x 96
55N. 924 nexttec™ 1-Step DNA Isolation Kit for Blood (10 µl)
24 nexttec™ cleanPlates96
24x 96

Protocols:

pdf55N-Blood10_1.0_nexttec-cleanColumns.pdf

pdf55N-Blood10_1.0_nexttec-cleanPlate96.pdf

 

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